KMID : 1011820230640010082
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Investigative and Clinical Urology 2023 Volume.64 No. 1 p.82 ~ p.90
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Effect of ¥á1D-adrenoceptor blocker for the reduction of ureteral contractions
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Hong Seong-Hwi
Jang Eun-Bi Park Sung-Yul Moon Hong-Sang Yoon Young-Eun Hwang Hyun-Ji
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Abstract
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Purpose : Urolithiasis is a common urinary tract disease with growing prevalence. Alpha1-adrenoceptors (¥á1-ARs) are abundant in ureteral smooth muscle, distributed with different ¥á1-AR subtypes. ¥á1D-AR is the most widely distributed in the ureter. However, the effect of ¥á1D-AR blockade on ureteric contraction remains unknown.
Materials and Methods : We dissected smooth muscle tissues (3 mm¡¿3 mm) from the rat bladder and human ureter, tied silk strips on both tissue ends, and measured contraction in an organ bath chamber. Contraction activity in ureteral smooth muscle cells (USMCs) was immunocytochemically examined using primary rat and human USMC cultures.
Results : Using the organ bath system, we determined the inhibitory effects of silodosin, tamsulosin, and naftopidil. Naftopidil significantly decreased contractility of rat bladder tissue; similar results were observed in human ureteral tissue. To confirm ex vivo experimental results in vitro , we examined the phosphorylation of myosin light chain (MLC), a marker of contractility, in a primary human USMC culture. The examined drugs decreased phospho-MLC levels in human USMCs; however, naftopidil profoundly increased MLC dephosphorylation.
Conclusions : We studied the effects of naftopidil, an ¥á1D-AR inhibitor, on the ureter. Compared with alpha-blockers, naftopidil significantly relaxed ureteral smooth muscle. Therefore, naftopidil could be an effective therapy for patients with ureteral stones.
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KEYWORD
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Alpha-adrenergic receptor, Smooth muscle, Ureter
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